Part:BBa_J100275:Design

tCloneTet+PDB riboswitch with P9

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 350
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 496
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 522
  Illegal NgoMIV site found at 890
  Illegal NgoMIV site found at 1050
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

Used PCR to "cut out" the P5 promoter since there were no restriction enzyme sites surrounding it.

Source

J100255